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KMID : 0368420220650040291
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Journal of Plant Biology
2022 Volume.65 No. 4 p.291 ~ p.310
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Comparative Analysis of miRNA Expression Profiles Provides Insight into Regulation of Biosynthesis of Flavonoids and Terpenoids Between Two Varieties of Toona sinensis Sprouts
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Zhao Hu
Shi Xiaopu
Shen Cheng
Chen Caifeng
Qu Changqing
Patel Gopal
Zhou Wei
Kai Guoyin
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Abstract
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In this study, small RNA sequencing has been performed to identify the miRNAs and explore their regulatory mechanism in T. sinensis sprouts. From HPLC analysis, the flavonoid content was higher in the BYC2 (purple toon sprout) than in the GYC2 (green toon sprout), whereas the volatile terpenoids revealed an inverse change. Therefore, the small RNA libraries of two varieties were constructed, and 331 known miRNAs and 23 novel miRNAs were discovered. Differential gene expression analysis demonstrated the upregulation of 25 miRNAs and the downregulation of 27 miRNAs between BYC2 and GYC2. qRT-PCR analysis showed that nine miRNAs involved in the biosynthetic regulation of flavonoids were downregulated in the BYC2, whereas eight miRNAs related to the regulation of the biosynthesis of terpenoids were upregulated, when compared with GYC2. The results indicated that the differential expression of the miRNAs mentioned above played a crucial role in the regulation of the formation of the flavonoids and terpenoids in T. sinensis sprouts. The present study not only fills in the paucity of knowledge regarding the T. sinensis miRNA, it also provides more valuable information for the genetic improvement of T. sinensis cultivation for the future.
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KEYWORD
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Flavonoids, Terpenoids, miRNA, T. sinensis, High-throughput sequencing, Post-transcriptional regulation
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